![]() Nuvia cPrime media are built on a rigid, mechanically and chemically stable macroporous base matrix with a particle size optimized to deliver exceptional flow properties, fast mass transfer, and stability. coli host cell proteins (ECP) and endotoxin levels. The Capto MMC ligand exhibits many functionalities for interaction with a target molecule. First, a capture step using Capto L was used to reduce E. Buffer B: 100 mM acetic acid, 50 mM Na-phosphate, 20 mM Na-succinate, pH 8. The ligand coupled to the resin surface allows electrostatic interactions as well as hydrophobic interaction, hydrogen bonding, or thiophilic interaction. More importantly, the media have a large design space for binding and elution, allowing for the development of highly robust methods in a commercial manufacturing setting. This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. Capto MMC 12 3 12 3 Column: Tricorn 5/100 Resin: (A) Capto MMC (B) SP Sepharose Fast Flow Sample: human blood plasma diluted 5 times, 10 CV Buffer A: 100 mM acetic acid, 50 mM Na-phosphate, 20 mM Na-succinate, pH 5.0. Examples of multimodal chromatography resins: (A) Capto MMC this resin is a mixed-mode cation exchanger with the properties of a weak cation exchanger. Nuvia cPrime media's unique selectivity allows method developers to use hydrophobic and cation exchange interaction modes to achieve effective purification. Comparable binding capacities (80-110 mg/mL), decreasing as salt is added, are observed for all three proteins on the Nuvia resins. These media are designed for process-scale purification of a wide variety of therapeutic proteins. Examples of multimodal chromatography resins: (A) Capto MMC this resin is a mixed-mode cation exchanger with the properties of a weak cation exchanger. Based on TEM imaging and iSEC, the Nuvia resins have a microgranular structure and large pores (110 nm radius), while the Capto resins have a fibrous structure and smaller pores (32-36 nm radius). Nuvia cPrime hydrophobic cation exchange media are a new addition to Bio-Rad's family of mixed-mode purification products. Capto MMC ImpRes (Fig 1) is a BioProcess chromatography medium (resin) for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.
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